KMID : 0881720140290010060
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Journal of Food Hygiene and Safety 2014 Volume.29 No. 1 p.60 ~ p.66
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Bactericidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient Against Pseudomonas Aeruginosa and Enterococcus Hirae
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Cha Chun-Nam
Park Eun-Kee Kim Yong-Pal Yu Eun-Ah Yoo Chang-Yeul Hong Il-Hwa Kim Suk Lee Hu-Jang
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Abstract
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This test was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 20% ortho-phenylphenol against Pseudomonas aeruginosa (P. aeruginosa) and Enterococcus hirae (E. hirae). In preliminary tests, P. aeruginosa and E. hirae working culture suspension number (N value) were 2.8 ¡¿ 108 and 4.0 ¡¿ 108CFU/mL, respectively. And all the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of P. aeruginosa and E. hirae recovered on the control-carriers (T value) was 2.8 ¡¿ 108 and 3.4 ¡¿ 106 CFU/mL, respectively. In the bactericidal effect of the fumigant, the reduction number of 2.8 ¡¿ 108 (d value) was 6.46 and 5.19 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results from this study, the fumigation disinfectant containing 20% ortho-phenylphenol has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with the pathogenic bacteria.
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KEYWORD
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Fumigation disinfectant, ortho-phenylphenol, Pseudomonas aeruginosa, Enterococcus hirae
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